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buffer 5mm kcl  (Biotium)


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    Biotium buffer 5mm kcl
    Buffer 5mm Kcl, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/buffer+5mm+kcl/bio_rxiv__64898__2026__05__07__723399-303-64-70?v=Biotium
    Average 94 stars, based on 222 article reviews
    buffer 5mm kcl - by Bioz Stars, 2026-07
    94/100 stars

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    A: Freshly fertilized eggs are transferred into a petri dish containing the test solution, viable eggs are selected microscopically, and 24 eggs containing viable embryos are transferred into a 24 well plates (1 egg per well). 20 wells contain 1 ml of BKI solution at a defined concentration (T), 4 wells serve as internal control (iC); B: 24 well-plate setup. T = BKI test concentration; nC = negative control <t>(E3</t> medium/osmosis water); iC = internal control (E3 medium/osmosis water); sC = solvent control (DMSO); pC = positive control: dichloroaniline (4 g/L in water) (adapted from [31; 34])
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    A: Freshly fertilized eggs are transferred into a petri dish containing the test solution, viable eggs are selected microscopically, and 24 eggs containing viable embryos are transferred into a 24 well plates (1 egg per well). 20 wells contain 1 ml of BKI solution at a defined concentration (T), 4 wells serve as internal control (iC); B: 24 well-plate setup. T = BKI test concentration; nC = negative control <t>(E3</t> medium/osmosis water); iC = internal control (E3 medium/osmosis water); sC = solvent control (DMSO); pC = positive control: dichloroaniline (4 g/L in water) (adapted from [31; 34])
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    A: Freshly fertilized eggs are transferred into a petri dish containing the test solution, viable eggs are selected microscopically, and 24 eggs containing viable embryos are transferred into a 24 well plates (1 egg per well). 20 wells contain 1 ml of BKI solution at a defined concentration (T), 4 wells serve as internal control (iC); B: 24 well-plate setup. T = BKI test concentration; nC = negative control <t>(E3</t> medium/osmosis water); iC = internal control (E3 medium/osmosis water); sC = solvent control (DMSO); pC = positive control: dichloroaniline (4 g/L in water) (adapted from [31; 34])
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    A: Freshly fertilized eggs are transferred into a petri dish containing the test solution, viable eggs are selected microscopically, and 24 eggs containing viable embryos are transferred into a 24 well plates (1 egg per well). 20 wells contain 1 ml of BKI solution at a defined concentration (T), 4 wells serve as internal control (iC); B: 24 well-plate setup. T = BKI test concentration; nC = negative control (E3 medium/osmosis water); iC = internal control (E3 medium/osmosis water); sC = solvent control (DMSO); pC = positive control: dichloroaniline (4 g/L in water) (adapted from [31; 34])

    Journal: International journal of antimicrobial agents

    Article Title: Comparative assessment of the effects of bumped kinase inhibitors on early zebrafish embryo development and pregnancy in mice.

    doi: 10.1016/j.ijantimicag.2020.106099

    Figure Lengend Snippet: A: Freshly fertilized eggs are transferred into a petri dish containing the test solution, viable eggs are selected microscopically, and 24 eggs containing viable embryos are transferred into a 24 well plates (1 egg per well). 20 wells contain 1 ml of BKI solution at a defined concentration (T), 4 wells serve as internal control (iC); B: 24 well-plate setup. T = BKI test concentration; nC = negative control (E3 medium/osmosis water); iC = internal control (E3 medium/osmosis water); sC = solvent control (DMSO); pC = positive control: dichloroaniline (4 g/L in water) (adapted from [31; 34])

    Article Snippet: At 3h post fertilization (3hpf), zebrafish eggs were transferred into a petri dish (Sarstedt Inc, Nümbrecht, Germany) containing E3-medium (5mM NaCl, 0.17mM KCl, 0.33mM CaCl 2 , and 0.33mM Mg SO 4 , buffered to pH7.5 using sodium-bicarbonate)/osmosis water) containing the tested drug concentration.

    Techniques: Concentration Assay, Negative Control, Positive Control